Cultivation of microalgae - Chlorella sorokiniana and Auxenochlorella protothecoides - in shrimp boiling water residues

Based on the ability of microalgae to purify industrial processing waters, overall aim this study was evaluate whether currently wasted shrimp waters could be used as microalgal growth media produce new protein-enriched food and feed ingredients. Low molecular weight (LMW) fractions boiling water (SBW) which had been pre-flocculated using alginate (AL), carrageenan (CA), chitosan (CH) or Superfloc C-592 recover protein via flotation, were for cultivation Chlorella sorokiniana Auxenochlorella protothecoides a biomass. CH-derived induced highest rates both species with A. out-performing C. sorokiniana. best assimilated phosphate-phoshorous (P-PO4) total phosphorous (TP) in all media; ?63 mg/L ? 45 after 4 days, respectively. In upscaled aerated cultures CH- AL-derived media, P TP uptake increased up 85 127 mg/L, Further, 63% free amino acids (AA) waters. Biomasses derived from SBW contained 37–43% 15.0–17.4% fatty (FA) per DW; 38–40% essential AA (EAA) 21.3–22.5% polyunsaturated FA (PUFA), respectively Corresponding numbers biomass cultivated control 11 53%, FA, respectively, 38% 15.6% EAA PUFA, Ability assimilate AA, generate protein-rich LMW-fractions thus revealed first time, paves way SBW-based biorefinery comprising chemical, physical microbial processes multiple products.